IMPACT OF COBALT-60 GAMMA IRRADIATION ON ADVENTITIOUS ROOT FORMATION AND BIOACTIVE COMPOUND ACCUMULATION IN Codonopsis javanica IN VITRO
DOI:
https://doi.org/10.62985/j.huit_ojs.vol26.no1E.339Keywords:
Adventitious root, bioactive compound, cobalt-60, Codonopsis javanica, gamma rayAbstract
This study examined the effects of gamma radiation on survival, root induction, and bioactive compound production in root biomass from leaf and node explants of Codonopsis javanica plantlets, aiming to support large-scale propagation. Survival and root induction in leaf explants decreased with higher radiation doses, with complete mortality at 40 Gy. However, polysaccharide and polyphenol contents at irradiation doses were 2.2 and 1.4 times higher than the control, respectively. Saponin accumulation was not significantly affected. In node explants, the highest survival and root induction occurred at 10 Gy and 20 Gy, with root numbers 1.6 times greater than the control. No roots formed at 50 Gy. The highest polyphenol and polysaccharide levels were observed at 10 Gy, while saponin content declined as irradiation increased.
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