DETERMINATION OF FOSFOMYCIN RESISTANT GENE (fosA3) IN β-LACTAMASE PRODUCING Escherichia coli FROM HOSPITAL WASTEWATER
DOI:
https://doi.org/10.62985/j.huit_ojs.vol26.no1E.341Keywords:
Fosfomycin resistant, fosA3 gene, ESBL-E. coli, hospital wastewaterAbstract
This study aims to determine the fosfomycin resistant gene (fosA3) from 22 ESBL-E. coli strains (11 E. coli strains carrying the blaCTX-M-1 gene and 11 strains carrying both genes (blaTEM + blaCTX-M-1)), which were isolated from treated wastewater at Vinh Long General Hospital (2023). In total, 22 strains had a fosfomycin (FO 200) resistance phenotype and 7 strains carried the fosA3 gene (accounting for 31.82%) and 15 strains did not contain fosA3 (68.18%). The characteristics of the seven ESBL-producing E. coli strains carrying the fosA3 gene were identified. Among them, five strains exhibited resistance to 9 of the 11 antibiotics tested and harbored three resistance genes (blaTEM, blaCTX-M-1, fosA3). In addition, two strains (TVL8 and TVL21) displayed resistance to 9 of the 11 antibiotics and simultaneously carried four resistance genes (blaTEM, blaCTX-M-1, mcr-1, fosA3). The presence of the fosA3 gene in wastewater environments highlights the risk of antibiotic resistance gene transmission within the community. This study emphasizes the necessity of controlling antibiotic use and monitoring resistance genetic elements to limit the development and spread of fosfomycin-resistant and other antibiotic-resistant bacterial strains.
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