EVALUATION OF ANTI-MELANOGENIC ACTIVITY OF PHYCOCYANIN EXTRACTED FROM Arthrospira platensis
DOI:
https://doi.org/10.62985/j.huit_ojs.vol26.no3.437Keywords:
Arthrospira platensis, phycocyanin, melanin, B16F10, cytotoxicityAbstract
Phycocyanin (CPC) is a powerful antioxidant protein extracted from Arthrospira platensis. This study extracted cosmetics-grade CPC from Arthrospira platensis and determined its melanin-inhibitory effect in vitro. Cell walls were disrupted by the freezing-thawing cycles in 0.1% or 1% CaCl2 solution, and then CPC was purified by ammonium sulfate to reach the cosmetic grade. In vitro cytotoxicity of CPC was evaluated in L-929 cells. The anti-melanogenic effect of CPC was performed in B16F10 cells. The B16F10 cells were cultured in the cell culture medium containing CPC at 1-10 µg/mL concentrations for 48 hours. Melanin content was determined by the ratio of OD420/mg protein. The results showed the optimized extraction method was the combination of 3 freezing-thawing cycles in 1% CaCl2 and two-step precipitation by ammonium sulfate (1st 20% and 2nd 30%) with the purity was 2.411 ± 0.090 and concentration was 3.84 ± 0.07 mg/mL. CPC is safe for L-929 cells, and IC50 was 1405 ± 16 µg/mL. CPC inhibited melanin synthesis in B16F10 cells at 5 µg/mL, and the melanin content decreased from 0.086 ± 0.012 to 0.022 ± 0.002. Conclusion: obtained cosmetic-grade CPC, and the CPC reduced melanin content at 5 µg/mL.
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